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Lowland Anoa has become endangered due to hunting and human activity. Protection and breeding of endangered species in a controlled environment is the best way of conservation. However, it is not possible to adopt this approach for all endangered species because of the cost involved and the ever-increasing number of critically endangered species. In consideration of these limitations to the conventional conservation methods, we established a primary cell culture of endangered buffalo Lowland Anoa, Bubalus quarlesi , for the preservation of this biological resource. The successful introduction of these three genes was confirmed by western blot with specific antibodies, and enzymatic activity.

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